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Formerly known as nutritionally variant streptococci, these organisms require pyridoxal (vitamin B6) to grow. They are frequent causative agents of culture-negative endocarditis. 2. Bacillus Species (Non-anthracis) clsi document m45 pdf
| Organism Group | Examples | Specific Testing Notes | | :--- | :--- | :--- | | | S. mitis , S. oralis | Requires supplementation with blood or lysed horse blood. | | HACEK Group | Aggregatibacter , Cardiobacterium | Incubation in CO2; often require 24-48 hours. | | Neisseria spp. | N. meningitidis (not gonorrhoeae) | Specific agar base and CO2 incubation. | | Gram-negative rods | Aeromonas , Plesiomonas , Vibrio | Standard media allowed, but unique breakpoints apply. | | Anaerobic Bacteria | Bacteroides , Clostridium , Fusobacterium | Broth microdilution in Brucella broth; specialized atmosphere. | | Campylobacter jejuni/coli | Enteric pathogens | Requires microaerophilic conditions; specific agar dilution methods. |
Precise temperatures (usually 35°C) and atmospheric conditions (ambient air vs. 5% CO2), alongside specific timing (20 to 48 hours). The official, authorized version of the CLSI M45
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Some medical libraries may loan a print copy, but PDFs cannot be legally shared due to DRM restrictions. Bacillus Species (Non-anthracis) | Organism Group | Examples
The document specifies unique QC organisms for each fastidious species. For example:
A critical foodborne pathogen requiring precise therapeutic monitoring.
Clinical microbiology laboratories frequently encounter common pathogens like Staphylococcus aureus or Escherichia coli . However, identifying and testing rare, slow-growing, or highly finicky organisms presents a significant clinical challenge.